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Leaf absorption characteristics can vary by water content, age, and other factors. all terms and abbreviations summarized in Table 1), relative water content (RWC), and leaf water potential (Ψ leaf; Jones, 2014). Hunt Jr, E. Raymond; Rock, Barrett N. - Detection of changes in leaf water content using Near- and Middle-Infrared reflectances: 1989 3 Hunt Jr, Raymond E.; Rock, Barrett N.; Nobel, Park S. - Measurement of leaf relative water content by infrared reflectance: 1987 Finally, leaf water potential is estimated using pressure–volume curve (PV) parameters. [9] For more accurate ETR values, the leaf absorption value and the ratio of PSII reaction centers to PSI reaction centers can be included in the equation. This index is a reflectance measurement that is sensitive to increasing leaf water content. However, the results were not evaluated on other After 2 h, the leaves were removed, the surface water was blotted-off and the turgid weight recorded. Water was then drained from the sand bed to stress the plants. To measure the moisture content present in leaves, take fresh leaves ,weigh them and let them to dry them in an oven, take readings of the dried leaves untill get the similar value in weight. Leaf relative water content was calculated using the following formula (Turner, 1981): The effect of K status on growth and drought resistance is discussed. The main effect of high K application on water relations was an increase in leaf water content and a slight decrease in leaf ψπ. Samples were then dried in an oven at 70°C to constant weight. leaves were immersed in distilled water in a Petri dish. All leaf water content indices examined exhibited basic correlations with the relative water content (RWC) of leaves, while the R 1300 /R 1450 leaf water index also demonstrated a high signal strength and low variability (R 2 >0.94). When the PM diffusive resistance reached about 1000 s m −1, relative water content (RWC) of the second leaf was determined. Leaf water content is one of the most common physiological parameters limiting efficiency of photosynthesis and biomass productivity in plants including Miscanthus. If absorption differences are a concern, absorption can be measured with the use of an integrating sphere . Leaf water content, typically measured as the weight of water per leaf area or equivalent water thickness (EWT), is an indicator of tree health that is affected by several tree stressors (Carter, 1993; Chaerle and Van Der Straeten, 2000). Then, relative water content is estimated, accounting for saturated water mass per leaf area (SWMA), which is the product of leaf dry mass per area (LMA) and saturated water content (SWC). Leaf diffusive resistance (LDR) was measured at 1000 and 1300 h each day as stress developed. Therefore, it is of great significance to determine or predict the water content quickly and non-destructively. tent (RWC) to leaf reflectance in the 1 300-2 400- nm spectral domain.Leaf relative water content is the actual leaf water content relative to the leaf water content at full turgescence.The relation-ships were fitted using several types of leaves, ranging from succulent to low water content leaves and provided good performances. As the water content of leaves in vegetation canopies increases, the strength of the absorption around 1599 nm increases. Leaf area was found to be an almost linear function of absolute leaf water content (that is fresh weight—dry weight) for several dicotyledonous genotypes. Absorption at 819 nm is nearly unaffected by changing water content… These indices are correlated for a given dehydrating leaf and provide different information. All examined leaf reflectance ratios could also be correlated with leaf thickness. 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